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Effect of polymerization conditions on morphology and chromatographic characteristics of polyacrylamide‐based beds (monoliths) for capillary electrochromatography and capillary liquid chromatography
Author(s) -
Ratautaite Vilma,
Maruška Audrius,
Erickson Magnus,
Kornyšova Olga
Publication year - 2009
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200800705
Subject(s) - capillary electrochromatography , electrochromatography , chromatography , capillary action , polyacrylamide , monolith , chemistry , capillary electrophoresis , polymerization , monolithic hplc column , high performance liquid chromatography , materials science , polymer , polymer chemistry , catalysis , organic chemistry , composite material
Pioneering research work on continuous or monolithic beds for LC was published in 1989 [1]. However, till date there is a lack of complex investigation of various factors affecting the morphology of the beds polymerized from water‐soluble co‐monomers. In this article the effect of buffer, used as polymerization medium, concentration and pH, and total monomer concentration on the morphology of the resultant continuous beds are discussed. Evaluation of the continuous beds was performed by means of capillary LC, CEC, and inverse size‐exclusion chromatography.