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Molecularly imprinted polymer for solid‐phase extraction of ephedrine and analogs from human plasma
Author(s) -
Lasáková Martina,
Thiébaut Didier,
Jandera Pavel,
Pichon Valérie
Publication year - 2009
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200800684
Subject(s) - molecularly imprinted polymer , ephedrine , chemistry , nip , chromatography , extraction (chemistry) , methacrylic acid , solid phase extraction , molecular imprinting , alkaloid , polymer , human plasma , monomer , selectivity , organic chemistry , materials science , pharmacology , composite material , catalysis , medicine
Abstract A molecularly imprinted polymer (MIP) was synthesized and evaluated to selectively extract ephedrine from human plasma. The MIP synthesis was performed in chloroform with methacrylic acid as a functional monomer and the target alkaloid as a template molecule. The resulting MIP was applied to the selective extraction of ephedrine from a pure aqueous medium. A recovery about 74% was obtained using the MIP with only 7% on the nonimprinted polymer (NIP). A very straightforward selective SPE procedure was then successfully applied to the direct extraction of ephedrine from spiked human plasma with a high extraction recovery (68%) on the MIP with no recovery on the NIP. Moreover, the MIP was used for the selective extraction of catecholamine neurotransmitters, i.e . adrenaline and noradrenaline.