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Quantitation of imidazo[1,2‐ a ]quinoxaline derivatives in human and rat plasma using LC/ESI‐MS
Author(s) -
Khier Sonia,
Moarbess Georges,
DeleuzeMasquefa Carine,
Solassol Isabelle,
Margout Delphine,
Pinguet Frédéric,
Bonnet PierreAntoine,
Bressolle Françoise M. M.
Publication year - 2009
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200800668
Subject(s) - chemistry , chromatography , analyte , metabolite , quinoxaline , pharmacokinetics , formic acid , pharmacology , biochemistry , medicine , organic chemistry
Since several years, our group developed quinoxalinic compounds. Among the synthesized compounds, in the imidazo[1,2‐ a ]quinoxaline series, EAPB0203 has shown interesting activities both on melanoma and lymphoma. The structure of EAPB0203 has been modulated and a new compound, EAPB0503, exhibits an in vitro cytotoxic activity on melanoma cancer cell line 7–9 times higher than EAPB0203. We validated an LC/ESI‐MS method to simultaneously quantify EAPB0503 and its metabolite EAPB0603 in human and rat plasma. Chromatography was performed on a C8 Zorbax eclipse XDB column with a mobile phase consisting of acetronitrile and formate buffer gradient elution. LC‐MS data were acquired in SIM mode at m/z 305, 291, and 303 for EAPB0503, EAPB0603, and the internal standard, respectively. The drug/internal standard peak area ratios were linked via quadratic relationships to concentrations (low range: 5–300 μg/L, high range: 100–1000 μg/L). The method is precise (precision, ⪇14%) and accurate (recovery, 92–113%). Mean extraction efficiencies, >72% for each analyte, were obtained. The lower LOQs were 5 μg/L. This highly specific and sensitive method was successfully used to investigate plasma concentrations of EAPB0503 and EAPB0603 in a pharmacokinetic study carried out in rat and would also be useful in clinical trials at a later stage.