Premium
Polymer monolith microextraction online coupled to hydrophilic interaction chromatography/mass spectrometry for analysis of β 2 ‐agonist in human urine
Author(s) -
Zheng MingMing,
Zhang ManYu,
Feng YuQi
Publication year - 2009
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200800644
Subject(s) - monolith , chromatography , mass spectrometry , chemistry , solid phase microextraction , urine , gas chromatography–mass spectrometry , organic chemistry , biochemistry , catalysis
An analytical method based on online combination of polymer monolith microextraction (PMME) technique with hydrophilic interaction LC (HILIC)/MS is presented. The extraction was performed with a poly(methacrylic acid‐ co ‐ethylene glycol dimethacrylate) monolithic column while the subsequent separation was carried out on a Luna silica column by HILIC. After 1:1 v/v dilution with 20 mM phosphate solution at pH 7.0 and centrifugation, urine sample was directly used for extraction. After optimization, 85% ACN (containing 0.3% formic acid v/v) was used for rapid online elution, which was also the mobile phase in HILIC to avoid band broadening during separation or carry‐over that was usually observed in PMME‐RP LC system. Online automation of extraction and separation procedures was realized under the control of a program in this study. The developed method was applied to rapid and sensitive monitoring of three β 2 ‐agonist traces in human urine. The LODs (S/N = 3) of the method were found to be 0.05–0.09 ng/mL of β 2 ‐agonists in urine. The recoveries of three β 2 ‐agonists spiked in five different urine samples ranged from 79.8 to 119.8%, with RSDs less than 18.0%.