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Accurate molecular weight analysis of histones using FFE and RP‐HPLC on monolithic capillary columns
Author(s) -
Sneekes EvertJan,
Han Jun,
Elliot Monica,
Ausio Juan,
Swart Remco,
Heck Albert J. R.,
Borchers Christoph
Publication year - 2009
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200800627
Subject(s) - histone , capillary electrophoresis , chromatography , monolithic hplc column , chemistry , capillary action , proteomics , dna , transcription (linguistics) , electrophoresis , computational biology , high performance liquid chromatography , biochemistry , biology , materials science , gene , composite material , linguistics , philosophy
Due to their large diversity with respect to post‐translational modifications (PTMs), the family of histones provides a major analytical challenge in current proteomics research. Their function has a large impact on the transcription of DNA, and as a result, on the expression of proteins. The variation in PTMs regulates transcription, and, as a result, many methods are being employed for the in‐depth analysis of histones. In this paper, we present a separation strategy for histones based on free‐flow electrophoresis (FFE) followed by an RP separation on capillary monolithic PS‐DVB columns. The capillary columns are directly interfaced with an FT‐ICR MS providing an online system for the detection and accurate molecular weight analysis of intact histones.