Premium
Zwitterionic molecularly imprinted polymer‐based solid‐phase micro‐extraction coupled with molecularly imprinted polymer sensor for ultra‐trace sensing of L ‐histidine
Author(s) -
Prasad Bhim Bali,
Tiwari Khushaboo,
Singh Meenakshi,
Sharma Piyush S.,
Patel Amit K.,
Srivastava Shrinkhala
Publication year - 2009
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200800595
Subject(s) - molecularly imprinted polymer , detection limit , solid phase extraction , analyte , polymer , chromatography , chemistry , solid phase microextraction , extraction (chemistry) , sorption , molecular imprinting , adsorption , selectivity , mass spectrometry , gas chromatography–mass spectrometry , organic chemistry , catalysis
The proposed L ‐histidine sensing system composed of a molecularly imprinted solid‐phase microextraction component combined with a molecularly imprinted polymer sensor was used to determine critical levels of test analyte in a complex matrix of highly diluted human blood serum without any non‐specific sorption and false‐positive contributions. The molecularly imprinted polymer was a zwitterionic polymer brush derived from the disodium salt of EDTA and chloranil, grafted to solid‐phase microextraction material. The hyphenated approach was able to detect L ‐histidine quantitatively with a limit of detection as low as 0.0435 ng/mL ( RSD = 0.2%, S/N = 3).