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Radial distribution of the contributions to band broadening of a silica‐based semi‐preparative monolithic column
Author(s) -
Abia Jude A.,
Mriziq Khaled S.,
Guiochon Georges A.
Publication year - 2009
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200800590
Subject(s) - column (typography) , analytical chemistry (journal) , chemistry , diffusion , mass transfer , eddy diffusion , silica gel , monolithic hplc column , chromatography , amperometry , materials science , electrode , electrochemistry , high performance liquid chromatography , mechanics , geometry , turbulence , thermodynamics , physics , mathematics , connection (principal bundle)
Abstract Using an on‐column local electrochemical microdetector operated in the amperometric mode, band elution profiles were recorded at different radial locations at the exit of a 10 mm id, 100 mm long silica‐based monolithic column. HETP plots were then acquired at each of these locations, and all these results were fitted to the Knox equation. This provided a spatial distribution of the values of the eddy diffusion ( A ), the molecular diffusion ( B ), and the resistance to the kinetics of mass transfer ( C ) terms. Results obtained indicate that the wall region yields higher A values and smaller C values than the central core region. Significant radial fluctuations of these contributions to band broadening occur throughout the exit column cross‐section. This phenomenon is due to the structural radial heterogeneity of the column.