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Poly( N,N ‐dimethylacrylamide‐ co ‐4‐(ethyl)‐morpholine methacrylamide) copolymer as coating for CE
Author(s) -
Bernal José,
SánchezHernández Laura,
Elvira Carlos,
Velasco Diego,
Ibáñez Elena,
Cifuentes Alejandro
Publication year - 2009
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200800575
Subject(s) - methacrylamide , copolymer , coating , morpholine , cationic polymerization , adsorption , chemistry , polymer chemistry , chemical engineering , materials science , polymer , organic chemistry , acrylamide , engineering
In this work, a new physically adsorbed coating for CE is presented. This coating is based on a poly( N,N ‐dimethylacrylamide‐ co ‐4‐(ethyl)‐morpholine methacrylamide) (DMA/MAEM) copolymer synthesized in our laboratory. It is demonstrated that the direction and magnitude of the EOF in CE can be modulated by varying the composition of the DMA/MAEM copolymer and the type and pH of the BGE. Moreover, the DMA/MAEM coating provides %RSD n = 5 values for migration times lower than 0.9% for the same capillary and day, whereas the %RSD n = 25 obtained for the interday assay was lower than 2.9%. The stability of the coating procedure is also tested between capillaries obtaining %RSD n = 15 values lower than 2.9%, demonstrating that this physically adsorbed copolymer gives rise to a stable and reproducible coating in CE. Finally, the usefulness of this new cationic copolymer as CE coating is demonstrated through different applications. Namely, it is demonstrated that the CE separation of basic proteins, nucleotides and organic acids is achieved in a fast and easy way by using the DMA/MAEM coated capillary. The use of fused bare silica capillaries did not allow the separation of these compounds under the same analytical conditions. These results demonstrate that this type of coating in CE provides the option of using BGEs that are useless when utilized together with bare silica capillaries making wider the application and possibilities of this analytical technique.