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HPLC enantioseparation of β 2 ‐homoamino acids using crown ether‐based chiral stationary phase
Author(s) -
Berkecz Róbert,
Ilisz István,
Misicka Aleksandra,
Tymecka Dagmara,
Fülöp Ferenc,
Choi Hee Jung,
Hyun Myung Ho,
Péter Antal
Publication year - 2009
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200800561
Subject(s) - enantiomer , chemistry , chromatography , elution , crown ether , high performance liquid chromatography , resolution (logic) , chiral derivatizing agent , analyte , phase (matter) , ether , chiral column chromatography , 18 crown 6 , chiral stationary phase , organic chemistry , molecule , ion , artificial intelligence , computer science
RP high‐performance liquid chromatographic methods were developed for the enantioseparation of eleven unusual β 2 ‐homoamino acids. The underivatized analytes were separated on a chiral stationary phase containing (+)‐(18‐crown‐6)‐2,3,11,12‐tetracarboxylic acid as chiral selector. The effects of organic (alcoholic) and acidic modifiers, the mobile phase composition and temperature on the separation were investigated. The structures of the substituents in the α‐position of the analytes substantially influenced the retention and resolution. The elution sequence was determined in some cases: the S enantiomers eluted before the R enantiomers.