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Preparative isolation of pseudolaric acids A and B, and their glucosides from the root bark of Pseudolarix kaempferi using high‐speed counter‐current chromatography
Author(s) -
Han QuanBin,
Wong Lina,
Lai Fanny,
Yang NianYun,
Song JingZheng,
Qiao ChunFeng,
Xu HongXi
Publication year - 2009
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200800547
Subject(s) - chemistry , countercurrent chromatography , bark (sound) , high performance liquid chromatography , glycoside , chromatography , methanol , solvent , diterpene , organic chemistry , physics , acoustics
In order to provide the chemical markers for the quality control of herbal medicines, four diterpenoids, pseudolaric acids A and B (PAA and PAB), and their glucosides were isolated from the methanol extract of the Chinese herb Pseudolarix kaempferi using high‐speed counter‐current chromatography (HSCCC). The diphase solvent system was n ‐hexane/EtOAc/MeOH/H 2 O which was used at two ratios (5:5:5:5 and 1:9:4:6 by volume) in the separation of pseudolaric acids and their glycosides, respectively. As a result, PAA (14 mg), PAB (129 mg), PAA‐ O ‐β‐ D ‐glucopyranoside (8 mg, PAAG), and PAB‐ O ‐β‐ D ‐glucopyranoside (42 mg, PABG) were obtained from 0.5 g of the crude extract. Their purities were determined to be above 97% by HPLC analysis. Their chemical structures were confirmed by 1 H and 13 C NMR analysis or HPLC comparison with the reference compounds.