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Application of centrifugal partition chromatography coupled with evaporative light scattering detection for the isolation of saikosaponins‐a and ‐c from Bupleurum falcatum roots
Author(s) -
Yoon Kee Dong,
Kim Jinwoong
Publication year - 2009
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200800544
Subject(s) - chemistry , chromatography , bupleurum , saponin , chromatography detector , butanol , methanol , solvent , column chromatography , ethyl acetate , high performance liquid chromatography , ethanol , organic chemistry , medicine , alternative medicine , pathology , traditional chinese medicine
Abstract Centrifugal partition chromatography (CPC) coupled with evaporative light scattering detection (ELSD) was applied to separate saikosaponins‐a and ‐c preparatively from Bupleurum falcatum roots. The two‐phase solvent system composed of ethyl acetate/ n ‐butanol/methanol/water (15:1:3:15 by volume) was used to yield saikosaponins‐a (36.1 mg) and ‐c (28.7 mg) from 370 mg of saponin‐rich extract. The purities of isolated compounds were 96.6 and 97.3% for saikosaponins‐a and ‐c, respectively. Structure identification of these compounds was accomplished by comparison of spectroscopic data of ESI‐MS, 1 H NMR, and 13 C NMR with those of previously reported values.