Premium
Extraction of antioxidants from plants using ultrasonic methods and their antioxidant capacity
Author(s) -
Adam Martin,
Dobiáš Petr,
Eisner Aleš,
Ventura Karel
Publication year - 2009
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200800543
Subject(s) - rutin , dpph , chemistry , chromatography , quercetin , coriandrum , sativum , extraction (chemistry) , high performance liquid chromatography , scopoletin , antioxidant , botany , organic chemistry , biology , medicine , alternative medicine , pathology
The analytical method based on the HPLC coupled with UV detection (HPLC‐UV) for the determination of selected antioxidants ( i.e . esculetin, scopoletin, 7‐hydroxycoumarine, rutin, xanthotoxin, 5‐methoxypsoralen and quercetin) in plant material was developed. Two ultrasonic extraction methods for the isolation of these compounds from the plants such as Mentha longifolia L., Mentha spicata L., Ruta graveolens L., Achyllea millefolium L., Plantago lanceolata L. and Coriandrum sativum L. were used. Both of these methods, i.e . ultrasonic probe and ultrasonic bath, were optimised and compared to each other. For the proposed HPLC‐UV method LOQ values in the range from 22.7 (xanthotoxin) up to 97.2 ng/mL (rutin) were obtained. For all extracts the antioxidant capacity based on the reduction of free 2,2‐diphenyl‐1‐picrylhydrazyl (DPPH) radical was also determined. Obtained results ranged from 10.11 up to 73.50% of DPPH radical inhibition.