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Covalent modification of fused silica capillaries with quaternized polyamines to achieve robust and stable anodic electroosmotic flow
Author(s) -
Stine Jesse J.,
Palmer Christopher P.
Publication year - 2009
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200800533
Subject(s) - surface modification , covalent bond , chemistry , polymer , iodide , chemical modification , anode , methyl iodide , polymer chemistry , chemical engineering , polyamine , organic chemistry , electrode , engineering , biochemistry
Synthetic chemistry originally developed for the manufacture of chemically stable silica polyamine composites was adapted for the modification of fused silica capillaries for application in CE. Polyethyleneimine (PEI) and polyallylamine (PAA) were covalently bonded to the interior surface of fused silica capillaries utilizing 3‐chloropropyltrichlorosilane (CPTCS) or 3‐glycidoxypropyl‐trimethoxysilane (GPTMS) to anchor the polymers to the surface. The surface‐bound polymers were subsequently quaternized using methyl iodide (MeI). The resulting modified capillaries were studied using CE, and were shown to provide reproducible, stable, and robust anodic EOF throughout the pH range of 2–10. Surface modifications utilizing CPTCS could be rinsed with up to 6 M HCL or 1 M NaOH without significant loss of surface modifier. The utility of the modified capillaries for the separation of simple anions and cations was demonstrated.