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Quantitative capillary electrophoresis and its application in analysis of alkaloids in tea, coffee, coca cola, and theophylline tablets
Author(s) -
Li Mengjia,
Zhou Junyi,
Gu Xue,
Wang Yan,
Huang Xiaojing,
Yan Chao
Publication year - 2009
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200800529
Subject(s) - theobromine , theophylline , caffeine , chromatography , capillary electrophoresis , chemistry , quantitative analysis (chemistry) , coca , reproducibility , medicine , pharmacology , psychiatry , endocrinology
A quantitative CE (qCE) system with high precision has been developed, in which a 4‐port nano‐valve was isolated from the electric field and served as sample injector. The accurate amount of sample was introduced into the CE system with high reproducibility. Based on this system, consecutive injections and separations were performed without voltage interruption. Reproducibilities in terms of RSD lower than 0.8% for retention time and 1.7% for peak area were achieved. The effectiveness of the system was demonstrated by the quantitative analysis of caffeine, theobromine, and theophylline in real samples, such as tea leaf, roasted coffee, coca cola, and theophylline tablets.