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Purification of flavonoids and triterpene saponins from the licorice extract using preparative HPLC under RP and HILIC mode
Author(s) -
Zhang Hui,
Guo Zhimou,
Li Wei,
Feng Jiatao,
Xiao Yuansheng,
Zhang Feifang,
Xue Xingya,
Liang Xinmiao
Publication year - 2009
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200800526
Subject(s) - hydrophilic interaction chromatography , chemistry , chromatography , triterpene , high performance liquid chromatography , elution , medicine , alternative medicine , pathology
A four‐channel preparative HPLC was employed to isolate and purify compounds from licorice extract. Two separation modes, RP and hydrophilic interaction LC (HILIC), were used in preparative HPLC. HILIC mode was adopted to resolve the purification of the compounds with similar hydrophobicity, which were co‐eluted under RP mode. Using the two separation modes during the purification process, fifteen compounds were isolated from licorice extract. The results indicated that preparative HPLC performed under HILIC mode is an efficient method for the isolation and purification of compounds from natural products.