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Polymer‐based monolithic columns in capillary format tailored by using controlled in situ polymerization
Author(s) -
Aoki Hiroshi,
Tanaka Nobuo,
Kubo Takuya,
Hosoya Ken
Publication year - 2009
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200800508
Subject(s) - monolith , polymer , capillary action , materials science , monolithic hplc column , polymerization , epoxy , monomer , miniaturization , in situ polymerization , fabrication , chromatography , nanotechnology , chemistry , composite material , organic chemistry , medicine , alternative medicine , pathology , catalysis
This review introduces to the readers our new perspectives of polymer‐based monolithic column with a high performance for small solutes such as drug candidates, illustrating the fabrication of LC columns in capillary. First, we briefly reviewed the status quo of polymer‐based monolithic columns, comparing with silica monoliths. The miniaturization of LC system with higher throughput (shorter analytical time) was stressed conceptually, along with a fine permeable bicontinuous monolithic structure with submicron domain size (skeletal thickness + pore size) for higher performance. Second, from these perspectives, our column preparation was described, while our specially designed porogenic solvents were introduced as a controller of the monolithic morphology via reaction‐induced phase separation. Specifically, monolithic columns were exemplified in two polymer formats, that is, one monolith prepared by free radical polymerization of glycerin 1,3‐dimethacrylate, GDMA, and the other prepared by stepwise polymerization of newly introduced multifunctional epoxy and diamino monomers. Both monolithic columns in capillary format demonstrated a fine bicontinuous structure, affording a good compatibility of the efficiency ( H ) and permeability ( D ). Especially, the epoxy‐based column showed an excellent separation impedance, E (= H 2 / D ). Our micro‐HPLC data were discussed along with a prototyped wired chip device.