Premium
Open tubular CEC in a microfluidic chip for rapid chiral recognition
Author(s) -
Zhang Guisen,
Qian Chungen,
Xu Youzhi,
Feng Xiaojun,
Du Wei,
Liu BiFeng
Publication year - 2009
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200800507
Subject(s) - microchannel , enantiomer , chemistry , avidin , microfluidics , chromatography , adsorption , fluorescence , chiral stationary phase , high performance liquid chromatography , analytical chemistry (journal) , nanotechnology , materials science , organic chemistry , biotin , biochemistry , physics , quantum mechanics
Chiral recognition of dansyl enantiomeric amino acids by microfluidic open tubular CEC (μOTCEC) with fluorescence detection was demonstrated. Avidin was employed as the chiral selector immobilized on the microchannel wall, which functioned as the chiral stationary phase (CSP) by physical adsorption. The condition of CSP on the glass wall was characterized using field emission SEM. Results indicated that avidin was homogenously distributed on the microchannel surface. Two parameters that played essential roles in μOTCEC for chiral recognition were investigated. Buffer pH could greatly change the amount of adsorption of avidin on the channel wall by altering the electrostatic attraction between them. Methanol, the organic additive to the running buffer, was also found significant for controlling the quality of the μOTCEC chiral separation by regulating the hydrophobic interaction between the enantiomers and the CSP. Under the optimized conditions, four dansyl racemic amino acids were then successfully separated by μOTCEC within 100 s with resolutions of 2.43, 1.88, 3.01 and 2.65 for dansyl‐Ser, dansyl‐Met, dansyl‐Thr and dansyl‐Val, respectively. Furthermore, a comparison with microfluidic CZE was investigated demonstrating that μOTCEC was a promising method for rapid chiral recognition.