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Selectivity differences between alkyl and polar‐modified alkyl phases in reversed phase high performance liquid chromatography
Author(s) -
Jing LinLin,
Jiang Ru,
Liu Peng,
Wang PingAn,
Shi TianYao,
Sun XiaoLi
Publication year - 2009
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200800468
Subject(s) - silanol , alkyl , chemistry , selectivity , polar , chromatography , phase (matter) , high performance liquid chromatography , chemical polarity , hydrophilic interaction chromatography , analytical chemistry (journal) , organic chemistry , molecule , physics , astronomy , catalysis
Compared to conventional C18 phases, polar‐modified phases have distinct differences with regards to chromatographic behavior. In the present study, ODS phases and polar‐modified phases were synthesized. The columns containing these new packings demonstrated satisfactory stability under both acidic (pH 1.5) and basic (pH 10) conditions. We evaluated the selectivity differences between alkyl and polar‐modified alkyl RP columns by using a range of neutral analytes. The polar‐modified alkyl phases showed excellent peak shapes for almost all compounds. We also compared the selectivity differences between them for separating nucleotides by using 100% aqueous mobile phase and tricyclic antidepressants in the intermediate pH mobile phases. The results demonstrated that polar‐modified phases display a significantly reduced hydrophobic nature and a significantly reduced silanol activity compared to the conventional C18 phases.