Premium
Proteomic analysis of striatal neuronal cell cultures after morphine administration
Author(s) -
BodzonKulakowska Anna,
Suder Piotr,
Mak Pawel,
BierczynskaKrzysik Anna,
Lubec Gert,
Walczak Beata,
Kotlinska Jolanta,
Silberring Jerzy
Publication year - 2009
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200800464
Subject(s) - proteome , morphine , proteomics , in vivo , chemistry , cell , oxidative stress , capillary electrophoresis , biological pathway , cell culture , biology , pharmacology , biochemistry , microbiology and biotechnology , gene expression , gene , genetics
Using primary neuronal cell culture assays, combined with 2‐D gel electrophoresis and capillary LC–MS, we identified differences in proteomes between control and morphine‐treated cells. Statistically significant differences were observed among 26 proteins. Nineteen of them were up‐regulated, while seven were down‐regulated in morphine‐treated cell populations. The identified proteins belong to classes involved in energy metabolism, associated with oxidative stress, linked with protein biosynthesis, cytoskeletal ones, and chaperones. The detected proteins demand further detailed studies of their biological roles in morphine addiction. It is crucial to confirm observed processes in vivo in order to reveal the nature and importance of the biological effect of proteome changes after morphine administration. Further investigations may lead to the discovery of new proteome‐based effects of morphine on living organisms.