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Metal ion analysis using microchip CE with chemiluminescence detection based on 1,10‐phenanthroline–hydrogen peroxide reaction
Author(s) -
Nogami Takahiro,
Hashimoto Masahiko,
Tsukagoshi Kazuhiko
Publication year - 2009
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200800448
Subject(s) - chemiluminescence , hydrogen peroxide , phenanthroline , chemistry , metal ions in aqueous solution , inorganic chemistry , metal , combinatorial chemistry , photochemistry , chromatography , organic chemistry
We developed a microchip CE method with chemiluminescence (CL) detection using the reaction of 1,10‐phenanthroline and hydrogen peroxide for separation and determination of metal ions, where the metal ions acted as catalysts for the CL reaction. The microchip consisted of two microchannels that crossed at the intersection and four reservoirs that accessed the ends of the channels. The metal ions in the sample solution migrated in the channel along with 1,10‐phenanthroline included in a running solution, and then mixed with hydrogen peroxide in one of the reservoirs to emit CL. The light was detected with a photomultiplier tube located just above the reservoir. Two metal ion groups, the platinum metal group (Ru(III), Rh(III), Pd(II), Os(VIII), Ir(III), and Pt(IV)) and the fourth periodic transition metal group (Cu(II), Fe(II), Co(II), and Ni(II)) were examined using the present system. The lowest detection limit was observed for Os(VIII); Os(VIII) responded over the range of 7.5×10 –12 –1.0×10 –8 M with the detection limit of 7.5×10 –12 M (about 38 zmol) (S/N = 3). The mixed solution of Ru(III), Rh(III), Pd(II), Os(VIII), Ir(III), and Pt(IV) could be analyzed using this system within about 2.5 min. In addition, the system was applied to the determination of Cu(II) concentration in a city water supply.