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Determination of adsorption isotherms by means of HPLC: Adsorption mechanism elucidation and separation optimization
Author(s) -
Marchetti Nicola,
Cavazzini Alberto,
Pasti Luisa,
Dondi Francesco
Publication year - 2009
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200800425
Subject(s) - adsorption , elution , chromatography , chemistry , high performance liquid chromatography , sorption isotherm , organic chemistry
Abstract The purpose of this review is to illustrate the most important techniques for isotherm determination by means of HPLC. Starting on the traditional Frontal Analysis approach, Frontal Analysis by Characteristic Point, Elution by Characteristic Point, Perturbation Method in its different applications will be considered to conclude with the most recent Inverse Method approach. Since many of these techniques are based on the fundamentals of nonlinear chromatography, a short overview of the theory of nonlinear chromatography is presented. Emphasis is given to the most recent applications of these techniques for pharmaceutical applications, characterization of binding mechanisms, bioaffinity studies, molecular and chiral recognition processes.