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Mobile phase additives for enhancing the chromatographic performance of astaxanthin on nonendcapped polymeric C 30 ‐bonded stationary phases
Author(s) -
Kaiser Philipp,
Surmann Peter,
Fuhrmann Herbert
Publication year - 2009
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200800408
Subject(s) - chemistry , elution , astaxanthin , chromatography , phase (matter) , methanol , high performance liquid chromatography , ether , triethylamine , ammonium acetate , ammonium , column chromatography , organic chemistry , food science , carotenoid
Astaxanthin shows peak deformation and reduced peak area response when eluted with methanol and methyl tert ‐butyl ether on nonendcapped polymeric C 30 ‐bonded HPLC phases. The present study tested different column manufacturers, column batches, and ten mobile phase additives including acids, bases, buffers, complexing and antioxidant agents for improvement of peak shape and peak area response. Concerning chromatographic benefits and feasibility, ammonium acetate was found to be the best additive followed by triethylamine for all columns tested. Variation of the mobile phase pH equivalent and the column temperature showed no synergistic effects on peak shape and peak area response. Results indicate that peak tailing and variation of peak area response are due to different on‐column effects. Possible mechanisms of the observed phenomenon will be discussed.