Premium
Preparative isolation and purification of two amides from Mallotus lianus Croiz by high‐speed counter‐current chromatography
Author(s) -
Jiang Liyan,
Lu Yanbin,
He Shan,
Pan Yuanjiang,
Sun Cuirong,
Wu Tianxing
Publication year - 2008
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200800396
Subject(s) - countercurrent chromatography , chromatography , chemistry , high performance liquid chromatography , ethyl acetate , solvent , organic chemistry
High‐speed counter‐current chromatography (HSCCC) was applied to the preparative isolation and purification of two amides from Mallotus lianus Croiz. In a single HSCCC separation, using the two‐phase solvent system composed of n ‐hexane/ethyl acetate/methanol/water (5:1:5:1 v/v), 247.5 mg of the enriched crude sample was separated to afford 10.3 mg of N ‐isobutyl‐2 E ,4 E ,12 Z ‐octadecatrienamide and 15.7 mg of (7 Z ,10 Z ,18 Z )tricosa‐7,10,18‐trienamide, a novel compound, with the purities of 98.0 and 94.6%, respectively. The HSCCC fractions were analyzed by HPLC and chemical structures of the compounds were identified by 1D‐ and 2D‐NMR, ESI‐, and GC‐MS.