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Reduction in delay time of high‐dwell volume pumps in LC‐MS applications using short‐term low‐ratio split flow
Author(s) -
Rogatsky Eduard,
Cruikshank Greg,
Stein Daniel T.
Publication year - 2009
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200800393
Subject(s) - dwell time , analyte , volume (thermodynamics) , reduction (mathematics) , retention time , volumetric flow rate , surface area to volume ratio , flow (mathematics) , splitter , chemistry , chromatography , computer science , mechanics , engineering , mathematics , physics , medicine , clinical psychology , geometry , quantum mechanics , chemical engineering
We present a simple hardware design which reduces run time of gradient‐based LC/MS applications and improves system equilibration. Our approach does not sacrifice efficiency of chromatographic separation, and does not affect analyte retention time and therefore does not require revalidation. Our technical design is based on a six‐port/two‐position switching valve and flow splitter installed prior to the LC column. This design minimizes time delays caused by the high‐dwell volume of some LC pumps. Implementation of short‐term (40–55 s) low‐ratio (1:10) flow splitting reduced delay times by over four‐fold in our application. This approach allowed hardware‐associated time delays to be minimized. Alternative plumbing suggestions are also discussed.