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Isolation of ATP from a yeast fermentation broth using a cryogel column at high flow velocities
Author(s) -
Yan Chen,
Shen Shaochuan,
Yun Junxian,
Wang Lianghua,
Yao Kejian,
Yao ShanJing
Publication year - 2008
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200800376
Subject(s) - fermentation , chromatography , elution , yeast , chemistry , raw material , ion exchange , adenosine triphosphate , ion chromatography , high performance liquid chromatography , biochemistry , ion , organic chemistry
This communication presents an effective method for isolating adenosine triphosphate (ATP) from a yeast fermentation broth using an anion‐exchange supermacroporous cryogel column at high flow velocities. The breakthrough and elution behaviors of pure ATP in the cryogel bed were investigated at flow velocities of 2, 5, and 10 cm/min and the ATP binding capacities were determined. Then the ATP‐containing yeast fermentation broth was employed as the test feedstock and various chromatographic runs were conducted to isolate ATP by the cryogel at different high flow velocities. The ATP samples obtained were analyzed quantitatively by HPLC. The results showed that even at a flow velocity of 5 or 10 cm/min, a product purity of 97.4 or 98.0% can be achieved, illustrating the potential of the present method for separation of high‐purity ATP directly from fermentation feedstock at high flow velocities.