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Determination of quinolone antibiotics in urine by capillary electrophoresis with chemiluminescence detection
Author(s) -
Liu YanMing,
Jia YuXiu,
Tian Wei
Publication year - 2008
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200800373
Subject(s) - capillary electrophoresis , chemiluminescence , quinolone , chromatography , chemistry , urine , antibiotics , biochemistry
A novel and simple method is presented for the determination of norfloxacin, ciprofloxacin, and ofloxacin by capillary electrophoresis with chemiluminescence detection. This method is based on the enhancing effect of quinolones on the chemiluminescence reaction of the Ce(SO 4 ) 2 –Ru(bpy) 3 2+ –HNO 3 system. Three quinolones were successfully separated and detected under optimum conditions. The obtained detection limits were 2.3×10 –7 mol/L, 5.2×10 –8 mol/L, and 7.8×10 –8 mol/L for ciprofloxacin, norfloxacin, and ofloxacin, respectively. The RSD of migration time and peak area were less than 1.8 and 3.8% ( n = 5), respectively. The applicability of the proposed method was illustrated in the determination of ofloxacin in eye drops and of norfloxacin in human urine samples, and the monitoring of pharmacokinetics for norfloxacin.