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Identification of collagen types in tissues using HPLC‐MS/MS
Author(s) -
Pataridis Statis,
Eckhardt Adam,
Mikulíková Katerina,
Sedláková Pavla,
Mikšík Ivan
Publication year - 2008
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200800351
Subject(s) - chromatography , chemistry , high performance liquid chromatography , mass spectrometry , identification (biology) , biology , botany
A method for the determination and quantification of collagen types I–V in rat tissues has been developed. This method is based on collagen fragmentation by cyanogen bromide followed by trypsin digestion. After that, HPLC‐MS/MS (HPLC coupled to an IT mass spectrometer) analyses of the resulting peptide mixtures (peptide maps) were performed. Specific peptides for each collagen type were selected. According to online databases, these peptides are present in human, bovine, and rat collagens. As a result, this method can be potentially applied to other species' tissues as well, such as human tissues, and provides a universal and simple method of quantifying collagen types. The applicability of this method for analyzing collagen types was demonstrated on rat tissues (skin, tendon, and aorta).