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Determination of amino acid neurotransmitters in human cerebrospinal fluid and saliva by capillary electrophoresis with laser‐induced fluorescence detection
Author(s) -
Deng YingHua,
Wang Hong,
Zhang HuaShan
Publication year - 2008
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200800339
Subject(s) - chemistry , derivatization , chromatography , capillary electrophoresis , taurine , analyte , glycine , fluorescence , glutamic acid , glutamine , fluorescein , micellar electrokinetic chromatography , amino acid , laser induced fluorescence , aspartic acid , cysteic acid , saliva , cerebrospinal fluid , high performance liquid chromatography , biochemistry , enzyme , medicine , physics , cystine , quantum mechanics , cysteine , pathology
A CE–LIF detection method has been developed to identify and quantitate six amino acid neurotransmitters including glutamic acid, aspartic acid, γ‐aminobutyric acid, glycine, taurine, and glutamine. N ‐Hydroxysuccinimidyl fluorescein‐ O ‐acetate, a fluorescein‐based dye, was employed for the derivatization of these neurotransmitters prior to CE–LIF analysis. Different parameters which influenced separation and derivatization were optimized in detail. Under optimum conditions, linearity was achieved within concentration ranges of up to three orders of magnitudes for those analytes with correlation coefficients from 0.9989 to 0.9998. The LODs ranged from 0.06 nM to 0.1 nM, and are thus superior or equivalent to those previously reported in the literature using CE–LIF detection. The proposed method has been successfully applied to the determination of amino acid neurotransmitters in biological samples such as human cerebrospinal fluid and saliva with satisfactory recoveries.