Premium
Determination of sulfonamides and trimethoprim using high temperature HPLC with simultaneous temperature and solvent gradient
Author(s) -
Giegold Sascha,
Teutenberg Thorsten,
Tuerk Jochen,
Kiffmeyer Thekla,
Wenclawiak Bernd
Publication year - 2008
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200800330
Subject(s) - high performance liquid chromatography , chromatography , chemistry , solvent , trimethoprim , viscosity , analytical chemistry (journal) , materials science , organic chemistry , biochemistry , antibiotics , composite material
A fast HPLC method for the analysis of eight selected sulfonamides (SA) and trimethoprim has been developed with the use of high temperature HPLC. The separation could be achieved in less than 1.5 min on a 50 mm sub 2 μm column with simultaneous solvent and temperature gradient programming. Due to the lower viscosity of the mobile phase and the increased mass transfer at higher temperatures, the separation could be performed on a conventional HPLC system obtaining peak widths at half height between 0.6 and 1.3 s.