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Supercritical fluid extraction for identification and determination of volatile metabolites from Angelica dahurica by GC‐MS
Author(s) -
Chen Qinhua,
Li Peng,
He Jing,
Zhang Zhuo,
Liu Jia
Publication year - 2008
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200800325
Subject(s) - chromatography , chemistry , imperatorin , supercritical fluid extraction , gas chromatography–mass spectrometry , extraction (chemistry) , mass spectrometry , supercritical fluid , gas chromatography , high performance liquid chromatography , organic chemistry
The volatile components of Angelica dahurica were obtained by supercritical fluid extraction (SFE) method. These oils obtained were analyzed by GC‐MS (identification and determination of metabolites). The compounds were identified according to their retention indices and mass spectra (electron impact (EI), 70 eV). The effects of different parameters, such as pressure, temperature, flow rate of CO 2 , and the amount of modifier, on the SFE of A. dahurica oil were investigated. A total of 50 compounds of SFE extracts were identified. Phellopterin (PO), isoimperatorin (IO), imperatorin (IM), alloimperatorin (AM), byakangelicin, isooxypeucedanin, and pimpinellin were the major coumarin compounds identified in A. dahurica SFE extracts. The quantitations of PO, IO, IM, and AM were then accomplished. The calibration curves showed good linearity ( R 2 >0.99) in the concentration ranges tested. The recoveries were higher than 85%, with RSDs less than 10%. The GC‐MS method was successfully validated and applied to the quantitation of A. dahurica .