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Fast adsorption and separation of bovine serum albumin and lysozyme using micrometer‐sized macromesoporous silica spheres
Author(s) -
Zhai Zheng,
Wang Yujun,
Chen Yang,
Luo Guangsheng
Publication year - 2008
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200800323
Subject(s) - adsorption , bovine serum albumin , chromatography , micrometer , lysozyme , protein adsorption , protein purification , chemistry , chemical engineering , spheres , volumetric flow rate , selective adsorption , materials science , analytical chemistry (journal) , organic chemistry , biochemistry , physics , astronomy , quantum mechanics , optics , engineering
Abstract Micrometer‐sized silica spheres with bimodal macromesoporous structures are synthesized continuously by a modified gelation technology, and a novel coaxial microfluidic device is applied to control the size of the silica spheres. These spheres are used as a new protein adsorbent to realize fast adsorption and separation of protein mixture. BSA and lysozyme (LYS) are as model proteins with different sizes and p I s. High protein adsorption capacity and rapid adsorption rate have been achieved. Fast separation of a binary mixture of BSA and LYS through a short packed column (50 mm×4.6 mm id) has also been successfully realized. LYS was selectively adsorbed while BSA flowed through the column in 12 s at a flow rate of up to 5.0 mL/min. As a result, an ultrafast adsorption and separation of BSA and LYS was obtained, showing great potential for applications in fast, large‐scale protein separation processes of these monodispersed silica spheres.