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Use of partially porous column as second dimension in comprehensive two‐dimensional system for analysis of polyphenolic antioxidants
Author(s) -
Dugo Paola,
Cacciola Francesco,
Herrero Miguel,
Donato Paola,
Mondello Luigi
Publication year - 2008
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200800281
Subject(s) - chromatography , column (typography) , porosity , chemistry , high performance liquid chromatography , pressure drop , wine , volumetric flow rate , two dimensional chromatography , monolithic hplc column , drop (telecommunication) , analytical chemistry (journal) , mechanics , mathematics , computer science , organic chemistry , telecommunications , physics , geometry , food science , connection (principal bundle)
In the present work, a comprehensive LC system using a microbore HPLC column in the first dimension and a partially porous column in the second dimension was developed and applied to the separation of polyphenolic components in a red wine sample. The performance of the partially porous short column (3.0 cm) was compared to that of a monolithic column, of comparable dimensions. The results obtained demonstrated the possibility to use partially porous columns to obtain fast analyses, using high flow rates, under repetitive gradient conditions and with very brief reconditioning times. A conventional HPLC system was used since the backpressure generated by the shell‐packed column, even at very high flow rates, was well within the operational limits. The use of an increased column temperature (60°C) allowed a further pressure‐drop decrease, with no stationary phase degradation, or loss in column performance.