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Evaluation of different internal‐diameter column combinations in comprehensive two‐dimensional gas chromatography in flavour and fragrance analysis
Author(s) -
Cordero Chiara,
Bicchi Carlo,
Galli Mario,
Galli Stefano,
Rubiolo Patrizia
Publication year - 2008
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200800280
Subject(s) - chromatography , chemistry , orthogonality , flavour , gas chromatography , two dimensional gas , fraction (chemistry) , two dimensional chromatography , homologous series , fractionation , column (typography) , analytical chemistry (journal) , mathematics , organic chemistry , geometry , food science , connection (principal bundle)
A series of OV1‐OV1701 column sets, in which the two dimensions differ in id and/or film thickness, were adopted to separate components of the volatile and semivolatile fraction of samples of plant origin. In particular two applications are presented: a target analysis approach to determine volatile suspected allergens in a medium‐complexity fragrance and in sandalwood essential oil, and a fingerprint analysis approach to compare herbal extracts. Some basic chromatographic parameters of these combinations, i. e. net separation measure ( S GC×GC ), degree of orthogonality and a suitable number of modulations per peak, were also estimated by analysing two test mixtures: FAME (C4:0–C24:0) and suspected volatile allergens. Experimental results show that 0.25 mm homologous id column combination, despite their lower separation power, have good capacity to separate and resolve medium‐to‐complex fractions from samples of plant origin, demonstrating: (i) good system orthogonality, maximized through proper exploitation of stationary‐phase selectivity and (ii) reduced 2‐D column overloading effects due to the increased 2‐D mass loadability, thus facilitating the analysis of mixtures whose components differ significantly in relative abundance.