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Multiparametric analysis of amino acids and organic acids in rat brain tissues using GC/MS
Author(s) -
Lin Jingchao,
Su Mingming,
Wang Xiaoyan,
Qiu Yunping,
Li Houkai,
Hao Jin,
Yang Hongzhou,
Zhou Mingmei,
Yan Chao,
Jia Wei
Publication year - 2008
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200800232
Subject(s) - brain tissue , chemistry , chromatography , quantitative analysis (chemistry) , amino acid , calibration curve , extraction (chemistry) , cerebral cortex , biomedical engineering , biochemistry , biology , detection limit , medicine , neuroscience
Using design of experiment (DOE) theory coupled with multivariate statistical analysis, we have developed a simple and reliable GC/MS‐based analytical assay for simultaneous analysis of amino acids and organic acids in rat brain tissue samples. The process of water extraction (pH 10.0) was extensively evaluated using brain tissue samples and a set of 21 reference standards. Acceptable calibration curves were obtained over a wide concentration range, 0.2–35.0 μg/mL for standards and 15.0–2.4 mL/g (tissue) for brain tissue samples. The precision was mostly better than 10% for both the mixed standards and the brain tissue samples. The brain tissue samples exhibited good stability within 48 h with RSD generally less than 15%. Furthermore, the developed analytical method was successfully applied in distinguishing the subtle variation among different parts of the brain tissues, such as cerebral cortex, hippocampus, and thalamus.