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Proteome analysis of human dorsolateral prefrontal cortex using shotgun mass spectrometry
Author(s) -
MartinsdeSouza Daniel,
Gattaz Wagner F.,
Schmitt Andrea,
Rewerts Christiane,
Maccarrone Giuseppina,
DiasNeto Emmanuel,
Turck Christoph W.
Publication year - 2008
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200800224
Subject(s) - proteome , shotgun , shotgun proteomics , dorsolateral prefrontal cortex , prefrontal cortex , nucleic acid , computational biology , proteomics , biochemistry , biology , chemistry , chromatography , neuroscience , cognition , gene
The prefrontal cortex executes important functions such as differentiation of conflicting thoughts, correct social behavior and personality expression, and is directly implicated in different neurodegenerative diseases. We performed a shotgun proteome analysis that included IEF fractionation, RP‐LC, and MALDI‐TOF/TOF mass spectrometric analysis of tryptic digests from a pool of seven human dorsolateral prefrontal cortex protein extracts. In this report, we present a catalog of 387 proteins expressed in these samples, identified by two or more peptides and high confidence search scores. These proteins are involved in different biological processes such as cell growth and/or maintenance, metabolism/energy pathways, cell communication/signal transduction, protein metabolism, transport, regulation of nucleobase, nucleoside, nucleotide and nucleic acid metabolism, and immune response. This analysis contributes to the knowledge of the human brain proteome by adding sample diversity and protein expression data from an alternative technical approach. It will also aid comparative studies of different brain areas and medical conditions, with future applications in basic and clinical research.