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Chemotaxonomy of bacteria by comprehensive GC and GC‐MS in electron impact and chemical ionisation mode
Author(s) -
David Frank,
Tienpont Bart,
Sandra Pat
Publication year - 2008
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200800215
Subject(s) - chemistry , gas chromatography–mass spectrometry , stenotrophomonas , chromatography , fatty acid methyl ester , gas chromatography , bacteria , chemical ionization , mass spectrometry , fatty acid , organic chemistry , biochemistry , biology , 16s ribosomal rna , ionization , ion , genetics , biodiesel , gene , catalysis
The analysis of the cellular lipidic fraction of bacteria is described. After hydrolysis and methylation, the fatty acid methyl esters (FAMEs) are determined by 1‐D GC using the Sherlock MIDI bacteria identification system, by comprehensive GC (GC×GC) and by GC‐MS in electron impact (EI) and positive chemical ionisation (PCI) mode. With GC×GC, the enhanced selectivity and group type separation provides a more complete elucidation of the fatty acids in microorganisms. GC‐EI‐MS and GC‐PCI‐MS were helpful for confirmation. The bacteria selected in this study are Brevundimonas diminuta , Chryseobacterium gleum and Stenotrophomonas maltophilia .