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Separation of inorganic anions on a high capacity porous polymeric monolithic column and application to direct determination of anions in seawater
Author(s) -
Evenhuis Christopher J.,
Buchberger Wolfgang,
Hilder Emily F.,
Flook Kelly J.,
Pohl Christopher A.,
Nesterenko Pavel N.,
Haddad Paul R.
Publication year - 2008
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200800205
Subject(s) - seawater , porosity , monolithic hplc column , chemistry , column (typography) , chromatography , chemical engineering , high performance liquid chromatography , organic chemistry , geology , oceanography , structural engineering , connection (principal bundle) , engineering
A commercially available 4.6 mm id×50 mm polymethacrylate‐based monolithic strong anion exchange column (ProSwift TM SAX‐1S) designed for the separation of proteins has been successfully used to separate small inorganic anions in the presence of a seawater sample matrix. Using a hydroxide eluent with suppressed conductivity detection the ion exchange capacity of this column declined over time; however, using KCl as the eluent, the column performance was stable with a capacity of 530 μequiv. for nitrate. The optimum conditions for the separation of iodate, bromate, nitrite, bromide and nitrate were assessed by constructing van Deemter plots using 1.00 and 0.100 M KCl. Efficiencies of up to 26 700 plates/m were recorded using 1.00 M KCl, at a flow rate of 0.20 mL/min but iodate was not baseline resolved from the void peak. By reducing the concentration of the eluent to 0.100 M, efficiencies of up to 39 900 plates/m could be obtained at 0.35 mL/min. By employing a linear gradient ranging from 0.05 to 1.00 M KCl the ions dissolved in distilled water or a salt water matrix could be baseline separated in less than 3 min at a flow rate of 2.50 mL/min.