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Capillary electrophoresis of some free fatty acids using partially aqueous electrolyte systems and indirect UV detection. Application to the analysis of oleic and linoleic acids in peanut breeding lines
Author(s) -
Bannore Yogita C.,
Chenault Kelly D.,
Melouk Hassan A.,
El Rassi Ziad
Publication year - 2008
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200800196
Subject(s) - capillary electrophoresis , chemistry , chromatography , linoleic acid , electrolyte , oleic acid , aqueous solution , peanut oil , electrophoresis , fatty acid , biochemistry , organic chemistry , raw material , electrode
Abstract This study has shown for the first time the suitability of CE with a partially aqueous electrolyte system for the analysis of free fatty acids (FFAs) in small portions of single peanut seeds. The partially aqueous electrolyte system consisted of 40 mM Tris, 2.5 mM adenosine‐5′‐monophosphate (AMP) and 7 mM α‐CD in ( N ‐methylformamide) NMF/dioxane/water (5:3:2 by volume) mixture, pH 8–9. While AMP served as the background UV absorber for indirect UV detection of the FFAs, the α‐CD functioned as the selectivity modulator by affecting the relative effective electrophoretic mobilities of the various FFAs due to their differential association with α‐CD. This CE method allowed the screening of peanut seeds for their content of oleic and linoleic acids, which is essential in breeding of peanuts of high‐oleic acid content. The extraction method of FFAs from peanut seeds is very reproducible with a high recovery approaching quantitative yield (∼97% recovery).