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Determination of dimethylarginine levels in rats using HILIC‐MS/MS: An in vivo microdialysis study
Author(s) -
Zotti Margherita,
Schiavone Stefania,
Tricarico Filomena,
Colaianna Marilena,
D'Apolito Oceania,
Paglia Giuseppe,
Corso Gaetano,
Trabace Luigia
Publication year - 2008
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200800147
Subject(s) - microdialysis , chemistry , hydrophilic interaction chromatography , asymmetric dimethylarginine , nitric oxide , chromatography , cerebrospinal fluid , in vivo , nitric oxide synthase , arginine , high performance liquid chromatography , biochemistry , extracellular , neuroscience , amino acid , psychology , organic chemistry , microbiology and biotechnology , biology
Nitric oxide (NO) is one of the most important mediators and neurotransmitters and its levels change under pathological conditions. NO production may be regulated by endogenous nitric oxide synthase (NOS) inhibitors, in particular asymmetric dimethylarginine (ADMA). Most of the interest is focused on ADMA, since this compound is present in plasma and urine and accumulation of ADMA has been described in many disease states but little is known about cerebrospinal fluid (CSF) concentrations of this compound and of its structural isomer symmetric dimethylarginine (SDMA). To determine the levels of methylarginines, we here present a new hydrophilic interaction chromatography (HILIC)‐MS/MS method for the precise determination of these substances in CSF from microdialysis samples of rat prefrontal cortex (PFC). The method requires only minimal sample preparation and features isotope‐labelled internal standards.