Premium
Determination of phenolic compounds derived from hydrolysable tannins in biological matrices by RP‐HPLC
Author(s) -
Díez María Teresa,
del Moral Pilar García,
Resines José Antonio,
Arín María Jesús
Publication year - 2008
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200800143
Subject(s) - chemistry , ellagic acid , chromatography , gallic acid , repeatability , pyrogallol , high performance liquid chromatography , resorcinol , polyphenol , tannin , phenols , food science , organic chemistry , antioxidant
An RP‐HPLC method for the determination of four phenolic compounds: gallic acid (GA), pyrogallol (PY), resorcinol (RE) and ellagic acid (EA), derived from hydrolysable tannins is reported. Separation was achieved on a SunFire C18 (250×4.6 mm id, 5 μm) column at 40°C with gradient elution. UV detection at 280 nm was applied. The developed method was validated in terms of linearity, accuracy and precision. Satisfactory repeatability and between day precision were noticed with RSD values lower than 3%. Recoveries from different biological samples ranged from 91.50 to 105.25%. The LODs were estimated as 1.70 mg/L for PY, 1.68 mg/L for GA, 1.52 mg/L for RE and 0.98 mg/L for EA with a 20 μL injection volume. The method was applied for the determination of these compounds in oak leaves and in ruminal fluid and urine samples taken from beef cattle fed with oak leaves. The proposed method could be used in ruminant nutrition studies to verify the effect that a diet rich in tannins have on ruminal fermentation and to determine the toxicity of these compounds.