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Evaluation of mobile phase, ion pairing, and temperature influence on an HILIC‐MS/MS method for L ‐arginine and its dimethylated derivatives detection
Author(s) -
Paglia Giuseppe,
D'Apolito Oceania,
Tricarico Filomena,
Garofalo Daniela,
Corso Gaetano
Publication year - 2008
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200800142
Subject(s) - hydrophilic interaction chromatography , chemistry , chromatography , arginine , analyte , high performance liquid chromatography , analytical chemistry (journal) , amino acid , biochemistry
Asymmetric N G ,– N G ‐dimethylarginine (ADMA) increases in diseases such as renal failure, diabetes mellitus, and hypercholesterolemia. The feasibility and utility of a hydrophilic interaction chromatography (HILIC) method for the separation of free L ‐arginine (Arg), ADMA, and symmetric N G ,– N G′ ‐dimethylarginine (SDMA) on a typical silica column were explored and the impact of some experimental parameters on the chromatographic behavior of these analytes was investigated. The effect of water and TFA content in mobile phase and of column temperature was investigated during the development of a fast and simple HILIC‐MS/MS method that might be suitable for the quantification of free Arg, ADMA, and SDMA in plasma for routine analysis. Our results show that a good compromise between efficiency and peak shape with acceptable retention and total chromatographic run time is achieved using an ACN/water (90:10) mobile phase with TFA% as additive ranging from 0.015 to 0.025% and column temperature ranging from 25 to 30°C.