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Preparative separation of dioscin derivatives from Dioscorea villosa by centrifugal partition chromatography coupled with evaporative light scattering detection
Author(s) -
Yoon Kee Dong,
Kim Jinwoong
Publication year - 2008
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200800136
Subject(s) - chemistry , chromatography , diosgenin , chloroform , saponin , dioscorea , glucoside , methanol , organic chemistry , medicine , alternative medicine , pathology
Dioscin derivatives from the Dioscorea villosa roots were isolated and purified by centrifugal partition chromatography coupled with evaporative light scattering detection. The two‐phase solvent system composed of chloroform‐methanol‐isopropanol‐water (7:6:1:4, v/v/v/v) was used to obtain prosapogenin A of dioscin ( 1 , 11.1 mg), dioscin ( 2 , 8.9 mg), deltonin ( 3 , 29.2 mg) and diosgenin 3‐ O ‐[α‐L‐rhamnopyranosyl(1 → 2)]‐[β‐D‐glucopyranosyl(1 → 3)‐β‐D‐glucopyranosyl(1 → 4)]‐β‐D‐glucopyranoside ( 4 , 6.2 mg) from 300 mg of saponin‐rich extract from the roots of D. villosa . The purities of 1 , 2 , 3 and 4 were determined to be 98.9, 97.4, 99.2 and 99.5%, respectively. Their chemical structures were determined by spectroscopic data of ESI‐MS, 1 H NMR and 13 C NMR and comparing with those of previously reported values.