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Simultaneous determination of thirteen plant alkaloids in a human specimen by SPE and HPLC
Author(s) -
Pietsch Jörg,
Günther Jana,
Henle Thomas,
Dreßler Jan
Publication year - 2008
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200800128
Subject(s) - chromatography , chemistry , brucine , harmine , cytisine , high performance liquid chromatography , alkaloid , aconitine , solid phase extraction , adulterant , anabasine , strychnine , pharmacology , organic chemistry , medicine , biochemistry , receptor , nicotinic agonist
A new screening method for the simultaneous determination of thirteen plant alkaloids (aconitine, anabasine, atropine, brucine, colchicine, cotinine, cytisine, harmine, ibogaine, nicotine, scopolamine, strychnine, yohimbine) in a human specimen was developed based on solid‐phase extraction and reversed‐phase liquid chromatography with photodiode array detection. The validated method enables selective identification as well as accurate and sensitive quantification. The analysis of forensic and clinical samples emphasizes the applicability for intoxications and drug abuse, as well as for compliance control.