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Brief analysis of the retention process in RP‐HPLC systems with a C 30 bonded stationary phase
Author(s) -
Zapała Wojciech
Publication year - 2008
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200800092
Subject(s) - high performance liquid chromatography , chemistry , chromatography , phase (matter) , stationary phase , adsorption , partition (number theory) , standard deviation , reversed phase chromatography , retention time , analytical chemistry (journal) , mathematics , statistics , organic chemistry , combinatorics
The influence of the mobile‐phase composition on the retention of eight model substances in different RP‐HPLC systems with a C 30 alkyl bonded stationary phase has been studied. The aim of this study was to compare the performance of four valuable retention models assuming the partition and adsorption mechanism of retention. All the models were verified for different experimental data by four criteria: the sum of squared differences between the experimental and theoretical data; the approximation of the standard deviation; the Fisher test; and the F‐test ratio.