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Application of the iodine–azide postcolumn reaction in RP‐HPLC for the determination of thioguanine in urine
Author(s) -
Zakrzewski Robert
Publication year - 2008
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200800056
Subject(s) - chemistry , chromatography , azide , high performance liquid chromatography , iodine , urine , detection limit , quantitative analysis (chemistry) , linear range , organic chemistry , biochemistry
The study focuses on the analysis of a sensitive, selective, and simple postcolumn detection method for thioguanine determination, based on the sensitizing induction of thioguanine on iodine–azide reaction and the combination technique of HPLC. The analysis was accomplished in the optimum conditions for iodine–azide detection system and HPLC separation. The values for the linear range, the LOD, and DOQ amounted to 0.8–1.7, 0.4, and 0.5 nmol/mL urine, respectively.