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Efficient enrichment of α,β‐unsaturated bovine insulins‐(C12) n using tweezing adsorptive bubble separation (TABS) with bovine serum albumin
Author(s) -
Nicolai Andreas,
Friess Albrecht,
Parlar Harun
Publication year - 2008
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200700660
Subject(s) - bovine serum albumin , chemistry , chromatography , bovine milk , aqueous solution , serum albumin , extraction (chemistry) , immunoassay , insulin , biochemistry , organic chemistry , antibody , medicine , biology , immunology
Alkyl‐substituted insulin molecules are depository preparations used in the treatment of diabetes. The isolation and purification of these active compounds is performed on the hand of several complicated separation steps. In this work, nonmodified and new synthesized α,β‐unsaturated bovine insulins‐(C12) n were selectively isolated from aqueous solution using tweezing adsorptive bubble separation (TABS) with BSA, which enabled reversible binding with the unpolar side chains of bovine insulin and its derivates. The bovine insulin/serum albumin complex could be efficiently transferred into the foam phase at pH 8 with an enrichment factor of 2.6 and a recovery of 89.7%. Experiments with alkylated α,β‐unsaturated bovine insulins‐(C12) n performed at pH 9 enriched even quantitatively the complex under equal experimental conditions, accompanied with an enrichment factor of 3.3. Additionally performed radio immunoassay tests revealed that the immunological activity of α,β‐unsaturated bovine insulins‐(C12) n remained unchanged after foaming.