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Chromatographic deuterium isotope effects of derivatized N ‐glycans and N ‐glycopeptides in a zwitterionic type of hydrophilic interaction chromatography
Author(s) -
Takegawa Yasuhiro,
Hato Megumi,
Deguchi Kisaburo,
Nakagawa Hiroaki,
Nishimura ShinIchiro
Publication year - 2008
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200700659
Subject(s) - chemistry , chromatography , deuterium , glycopeptide , high performance liquid chromatography , mass spectrometry , glycan , succinic anhydride , isotope , hydrophilic interaction chromatography , derivatization , organic chemistry , glycoprotein , biochemistry , physics , quantum mechanics , antibiotics
HPLC/MS is now an essential method for the analysis of glycans and glycopeptides. A technique using ICATs is also becoming popular for their comparative/quantitative analysis based on MS signal intensities. However, the RP HPLC most often used causes “doublet” peaks of deuterium‐labeled and nonlabeled peptides, which are well known for causing the chromatographic deuterium isotope effect. Such doublet peaks are undesirable for precise comparison of MS signal intensities. This report shows that, in the zwitterionic type of hydrophilic interaction chromatography, the chromatographic deuterium isotope effect is negligibly small for N ‐glycans derivatized with deuterium‐labeled 2‐aminopyridine and N ‐glycopeptides derivatized with deuterium‐labeled succinic anhydride.