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HILIC for separation of co‐eluted flavonoids under RP‐HPLC mode
Author(s) -
Zhang Hui,
Guo Zhimou,
Zhang Feifang,
Xu Qing,
Liang Xinmiao
Publication year - 2008
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200700656
Subject(s) - chromatography , chemistry , elution , high performance liquid chromatography , hydrophilic interaction chromatography
Hydrophilic interaction chromatography (HILIC) was employed to separate the co‐eluted flavonoids from licorice extract under RP‐HPLC mode. HILIC separations were carried out with the Atalantis HILIC Silica column and the CD‐based column. The co‐eluted flavonoids were well retained and separated on the two HILIC columns under HILIC mode. Similar results were obtained in the separation of another isoflavones sample, from kudzu extract under HILIC mode.