Premium
Hydrophilic interaction and reversed‐phase ultra‐performance liquid chromatography TOF‐MS for metabonomic analysis of Zucker rat urine
Author(s) -
Gika Helen G.,
Theodoridis Georgios A.,
Wilson Ian D.
Publication year - 2008
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200700644
Subject(s) - chromatography , chemistry , hydrophilic interaction chromatography , urine , reversed phase chromatography , high performance liquid chromatography , biochemistry
Hydrophilic interaction chromatography (HILIC) provides a complementary technique to RP methods for the retention of polar analytes for LC‐MS‐based metabonomic studies. Combining the advantages of both RP and HILIC separations with the efficient and rapid separations obtained using sub‐2 μm particles via the recently introduced ultra‐performance LC (UPLC) enables increased coverage of the metabolites present in biological samples to be achieved. Here an HILIC‐UPLC‐MS method was developed to provide metabolite profiles for urine samples obtained from male Zucker rats. The resulting data were compared with results obtained for the same samples by RP‐UPLC‐MS and demonstrated the complementary nature of the two separations with both methods enabling discrimination between the different sample types. Interestingly sample type differentiation was based on different markers.