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Single‐step approach to β‐cyclodextrin‐bonded silica as monolithic stationary phases for CEC
Author(s) -
Hsieh MingLung,
Li GuanYi,
Chau LaiKwan,
Hon YungSon
Publication year - 2008
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200700631
Subject(s) - mesoporous material , adsorption , monolithic hplc column , mesoporous silica , desorption , chemistry , silica gel , elution , porosity , cyclodextrin , chromatography , scanning electron microscope , silicon , sol gel , analytical chemistry (journal) , materials science , high performance liquid chromatography , nanotechnology , catalysis , organic chemistry , composite material
A novel single‐step sol–gel approach for the preparation of β‐CD‐bonded silica monolithic electrochromatographic columns is established. The porous silica networks were fabricated inside fused‐silica capillaries using sol–gel processing of tetramethoxysilane and an organfunctional silicon alkoxide that contains β‐CD. Scanning electron micrographs and nitrogen adsorption–desorption data showed that these functional monolithic columns have double pores structures with micrometer‐size co‐continuous through‐pores and silica skeletons with open mesopores. The β‐CD monolithic columns have successfully been applied to the separation of several neutral and negatively charged isomers by CEC. The column performance was evaluated by using positional isomers of naphthalenedisulfonic acid as model compounds. A plate height of less than 10 μm for the first eluted isomer of naphthalenedisulfonic acid was obtained at an optimal flow rate (0.47 mm/s) of the mobile phase. Moreover, the columns have been proved to be stable for more than 100 runs during 3 months period and show reasonable column reproducibility.